Western Blot
EzWay Western Blot Kit
EzWay Western Blot Kit is designed for colorimetric detection of proteins captured on immunoblots using DAB or TMB.
These kits provided optimized all reagents for quick and convenient western blot assay.
Features
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Specific and accurate colorimetric protein detection
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Optimized all reagents supplied for quick and convenient western blot assay
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High sensitivity and clear background
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Easy to use
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DAB or TMB option
Kit Components
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25X Transfer Buffer (Tris-Glycine)
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6X Blocking Solution (3% Casein/PBS)
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PBS Powder Pouch
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Tween-20 (50%)
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Substrate solution (DAB or TMB)
Ordering information
PVDF Transfer Membrane
EzWay PVDF (Polyvinylidene Fluoride) membrane is provided for western blotting. It has strong binding affinity eliminating sample "pass-through the membrane". Pre-Cut Blotting Membranes are provided as membrane/filter paper sandwiches and ready to use for transfer and blotting experiment.
Features
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Naturally hydrophobic unsupported membrane
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Chemical compatibility allows the use of all commonly used stains
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Low backgrounds, high sensitivity
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Strong binding affinity eliminating sample "pass-through the membrane"
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BSA binding capacity is 215 ug/cm2
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Membrane Pore size : 0.22um
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Filter Material: Hydrophobic PVDF
Ordering information
Nitrocellulose Transfer Membrane
EzWay Nitrocellulose membrane is provided for western blotting. Pre-Cut Blotting Membranes are provided as membrane/filter paper sandwiches and ready to use for transfer and blotting experiment.
Features
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Precut Nicrocellulose transfer membrane and filter paper
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For procedures that require optimum resolution
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Membrane of choice for all protein or immunoblotting applications
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Cellulose acetate free - assuring high binding and sensitivity
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Nitrocellulose wets out naturally, does not require methanol, and will not turn hydrophobic during the transfer process
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Low background
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Easily blocked
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Membrane Pore size : 0.45um
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Dimensions : 8.2cm x 7.4cm
Ordering information
Secondary Antibody
In Western blotting, the secondary antibody is used to detect the primary antibody, which has already bound to the target protein. Horseradish peroxidase (HRP) conjugated secondary antibodies are the most common secondaries used for western blotting.